细胞增殖/凋亡

实验内容:

1、用marker笔在6孔板背后,均匀地划横线,间距0.5~1cm。

2、在空中加入约5X10^5个细胞,具体数量因细胞而异,过夜能铺满即可。

3、第二天用枪头垂直于背后的横线划痕,枪头竖直直。

4、用PBS洗细胞3次,去除划下的细胞,加入无血清培养基。

5、放入37℃5%CO2培养箱培养。按0、6、12、24小时取样,拍照。

实验结果:

1. 细胞计数结果

2. 细胞显微照片

3. 实验报告(包括材料、仪器、操作流程、结果)。

收费标准:

1800元/6孔板

周期:

预付款后2-4周,视细胞培养情况。


​The wound-healing assay is one of the earliest developed methods to studycell migration in vitro . This method is based on observation of cell migrationinto a “wound” that is created on a cell monolayer. Although not an exactduplication of cell migration in vivo, this method mimics to some extent migrationof cells in wound healing. 
In comparison with other popular in vitro methods, such as time-lapse microscopyand Boyden chamber assays, the wound-healing assay is particularly suitablefor studies of directional cell migration and its regulation by cell interaction with extracellular matrix (ECM) and cell–cell interactions. More recently, this assay also has been combined with microinjection or transfection to assess the effects of expression of exogenous genes on migration of individual cells (6–8). This is probably the simplest method to study cell migration in vitro and the only that requires commonand inexpensive supplies found in most labs that are capable of cell culturing.

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